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A B2m-NOG mouse is placed in the restrainer, and the tail is placed close to the thermal lamp (~10 cm distance). The restrainer is secured by a stand, and the tail is sterilized with 70% ethanol using a cotton swab. Using a <t>fiber</t> <t>optic</t> <t>light</t> <t>source</t> for easier visualization of the veins, 10 × 10 6 PBMCs are injected intravenously into a lateral tail vein.
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A B2m-NOG mouse is placed in the restrainer, and the tail is placed close to the thermal lamp (~10 cm distance). The restrainer is secured by a stand, and the tail is sterilized with 70% ethanol using a cotton swab. Using a fiber optic light source for easier visualization of the veins, 10 × 10 6 PBMCs are injected intravenously into a lateral tail vein.

Journal: Bio-protocol

Article Title: PBMC-Humanized Mouse Model for Multiple Sclerosis: Studying Immune Changes and CNS Involvement

doi: 10.21769/BioProtoc.5312

Figure Lengend Snippet: A B2m-NOG mouse is placed in the restrainer, and the tail is placed close to the thermal lamp (~10 cm distance). The restrainer is secured by a stand, and the tail is sterilized with 70% ethanol using a cotton swab. Using a fiber optic light source for easier visualization of the veins, 10 × 10 6 PBMCs are injected intravenously into a lateral tail vein.

Article Snippet: Fiber optic light source (Nikon, model: 72306) 12.

Techniques: Injection